anti-cd11c-pe cy7 Search Results


anti–cd11c-pe/cy7 (bu15)  (Thermo Fisher)
90
Thermo Fisher anti–cd11c-pe/cy7 (bu15)
(A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, <t>CD11c,</t> CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.
Anti–Cd11c Pe/Cy7 (Bu15), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti–cd11c-pe/cy7 (bu15)/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
anti–cd11c-pe/cy7 (bu15) - by Bioz Stars, 2026-04
90/100 stars
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anti cd11c pe cy7 n418  (Miltenyi Biotec)
96
Miltenyi Biotec anti cd11c pe cy7 n418
(A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, <t>CD11c,</t> CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.
Anti Cd11c Pe Cy7 N418, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd11c pe cy7 n418/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
anti cd11c pe cy7 n418 - by Bioz Stars, 2026-04
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pe-cy7-anti-cd11c n418  (Thermo Fisher)
90
Thermo Fisher pe-cy7-anti-cd11c n418
(A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, <t>CD11c,</t> CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.
Pe Cy7 Anti Cd11c N418, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe-cy7-anti-cd11c n418/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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anti-cd11c-pe-cy7  (Becton Dickinson)
90
Becton Dickinson anti-cd11c-pe-cy7
(A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, <t>CD11c,</t> CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.
Anti Cd11c Pe Cy7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd11c-pe-cy7/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
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pe cy7 anti cd11c  (Thermo Fisher)
86
Thermo Fisher pe cy7 anti cd11c
(A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, <t>CD11c,</t> CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.
Pe Cy7 Anti Cd11c, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe cy7 anti cd11c/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
pe cy7 anti cd11c - by Bioz Stars, 2026-04
86/100 stars
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anti-cd11c-pe-cy7  (Thermo Fisher)
90
Thermo Fisher anti-cd11c-pe-cy7
(A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, <t>CD11c,</t> CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.
Anti Cd11c Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd11c-pe-cy7/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
anti-cd11c-pe-cy7 - by Bioz Stars, 2026-04
90/100 stars
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anti-cd80 af647  (Thermo Fisher)
90
Thermo Fisher anti-cd80 af647
(A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, <t>CD11c,</t> CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.
Anti Cd80 Af647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd80 af647/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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cd11c-pe-cy7  (Thermo Fisher)
90
Thermo Fisher cd11c-pe-cy7
RSV-induced airway immune infiltration is reduced in TLR7 KO mice. WT C57BL/6 or TLR7 KO mice were infected with RSV (0.5-2x10 7 PFUs) or PBS via intranasal administration and analysis performed after 4, 7 or 42 dpi. (A) Airway inflammation was assessed by counting the total number of live cells isolated from the bronchoalveolar lavage (BAL) fluid. Data are expressed as mean ± SEM, n = 12-16 mice per experimental group from three independent experiments. Immune cell populations in the airways collected in the BAL fluid were determined by flow cytometry. (B) Innate immune cell types: interstitial macrophages (CD11b + F4/80 + ), patrolling monocytes (CD11b + Ly6C lo ), inflammatory monocytes (CD11b + Ly6C hi ), NK cells (CD3 - NK1.1 + ), neutrophils (CD11b + Ly6G + ), plasmacytoid DCs <t>(CD11c</t> + CD11b - PDCA-1 + ) and myeloid DCs (CD11c + CD11b + MHCII + ). Numbers of differentially stained eosinophils was determined by counting 500 cells from random fields by standard morphological criteria relative to the total number of isolated cells. (C) Adaptive T cell subtypes: T helper (CD3 + CD4 + ) and cytotoxic T cells (CD3 + CD8 + ). Cell populations were measured as absolute number of CD45 + population per total live cells. Data are expressed as mean ± SEM, n = 5-8 mice per experimental group from two independent experiments. Statistical analysis was conducted using two-way ANOVA test followed by Tukey’s post hoc test for multiple comparison test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001).
Cd11c Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd11c-pe-cy7/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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anti-cd40  (Thermo Fisher)
90
Thermo Fisher anti-cd40
RSV-induced airway immune infiltration is reduced in TLR7 KO mice. WT C57BL/6 or TLR7 KO mice were infected with RSV (0.5-2x10 7 PFUs) or PBS via intranasal administration and analysis performed after 4, 7 or 42 dpi. (A) Airway inflammation was assessed by counting the total number of live cells isolated from the bronchoalveolar lavage (BAL) fluid. Data are expressed as mean ± SEM, n = 12-16 mice per experimental group from three independent experiments. Immune cell populations in the airways collected in the BAL fluid were determined by flow cytometry. (B) Innate immune cell types: interstitial macrophages (CD11b + F4/80 + ), patrolling monocytes (CD11b + Ly6C lo ), inflammatory monocytes (CD11b + Ly6C hi ), NK cells (CD3 - NK1.1 + ), neutrophils (CD11b + Ly6G + ), plasmacytoid DCs <t>(CD11c</t> + CD11b - PDCA-1 + ) and myeloid DCs (CD11c + CD11b + MHCII + ). Numbers of differentially stained eosinophils was determined by counting 500 cells from random fields by standard morphological criteria relative to the total number of isolated cells. (C) Adaptive T cell subtypes: T helper (CD3 + CD4 + ) and cytotoxic T cells (CD3 + CD8 + ). Cell populations were measured as absolute number of CD45 + population per total live cells. Data are expressed as mean ± SEM, n = 5-8 mice per experimental group from two independent experiments. Statistical analysis was conducted using two-way ANOVA test followed by Tukey’s post hoc test for multiple comparison test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001).
Anti Cd40, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cd40/product/Thermo Fisher
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apc-conjugated anti-cd206  (Becton Dickinson)
90
Becton Dickinson apc-conjugated anti-cd206
RSV-induced airway immune infiltration is reduced in TLR7 KO mice. WT C57BL/6 or TLR7 KO mice were infected with RSV (0.5-2x10 7 PFUs) or PBS via intranasal administration and analysis performed after 4, 7 or 42 dpi. (A) Airway inflammation was assessed by counting the total number of live cells isolated from the bronchoalveolar lavage (BAL) fluid. Data are expressed as mean ± SEM, n = 12-16 mice per experimental group from three independent experiments. Immune cell populations in the airways collected in the BAL fluid were determined by flow cytometry. (B) Innate immune cell types: interstitial macrophages (CD11b + F4/80 + ), patrolling monocytes (CD11b + Ly6C lo ), inflammatory monocytes (CD11b + Ly6C hi ), NK cells (CD3 - NK1.1 + ), neutrophils (CD11b + Ly6G + ), plasmacytoid DCs <t>(CD11c</t> + CD11b - PDCA-1 + ) and myeloid DCs (CD11c + CD11b + MHCII + ). Numbers of differentially stained eosinophils was determined by counting 500 cells from random fields by standard morphological criteria relative to the total number of isolated cells. (C) Adaptive T cell subtypes: T helper (CD3 + CD4 + ) and cytotoxic T cells (CD3 + CD8 + ). Cell populations were measured as absolute number of CD45 + population per total live cells. Data are expressed as mean ± SEM, n = 5-8 mice per experimental group from two independent experiments. Statistical analysis was conducted using two-way ANOVA test followed by Tukey’s post hoc test for multiple comparison test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001).
Apc Conjugated Anti Cd206, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apc-conjugated anti-cd206/product/Becton Dickinson
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apc-conjugated anti-cd206 - by Bioz Stars, 2026-04
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anti-cd11b a647  (Thermo Fisher)
90
Thermo Fisher anti-cd11b a647
RSV-induced airway immune infiltration is reduced in TLR7 KO mice. WT C57BL/6 or TLR7 KO mice were infected with RSV (0.5-2x10 7 PFUs) or PBS via intranasal administration and analysis performed after 4, 7 or 42 dpi. (A) Airway inflammation was assessed by counting the total number of live cells isolated from the bronchoalveolar lavage (BAL) fluid. Data are expressed as mean ± SEM, n = 12-16 mice per experimental group from three independent experiments. Immune cell populations in the airways collected in the BAL fluid were determined by flow cytometry. (B) Innate immune cell types: interstitial macrophages (CD11b + F4/80 + ), patrolling monocytes (CD11b + Ly6C lo ), inflammatory monocytes (CD11b + Ly6C hi ), NK cells (CD3 - NK1.1 + ), neutrophils (CD11b + Ly6G + ), plasmacytoid DCs <t>(CD11c</t> + CD11b - PDCA-1 + ) and myeloid DCs (CD11c + CD11b + MHCII + ). Numbers of differentially stained eosinophils was determined by counting 500 cells from random fields by standard morphological criteria relative to the total number of isolated cells. (C) Adaptive T cell subtypes: T helper (CD3 + CD4 + ) and cytotoxic T cells (CD3 + CD8 + ). Cell populations were measured as absolute number of CD45 + population per total live cells. Data are expressed as mean ± SEM, n = 5-8 mice per experimental group from two independent experiments. Statistical analysis was conducted using two-way ANOVA test followed by Tukey’s post hoc test for multiple comparison test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001).
Anti Cd11b A647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, CD11c, CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.

Journal: The Journal of Clinical Investigation

Article Title: ILC2-modulated T cell–to-MDSC balance is associated with bladder cancer recurrence

doi: 10.1172/JCI89717

Figure Lengend Snippet: (A) Representative example of ILC labeling. Cells were gated on live lineage-negative CD127+ leukocytes (with lineage including CD3, CD14, CD56, CD19, CD11c, CD303a, CD15, CD203c, and FcεR1α). (B) Quantification of ILC1 (Lin–CD127+CRTH2–c-Kit–), ILC2 (Lin–CD127+CRTH2+), and ILC3 (Lin–CD127+CRTH2–c-Kit+) among urine cells (n = 23 samples). (C) Absolute number of ILC2 in post-BCG samples. (D) Cocultures of PBMCs from HDs (n = 6) with the NMIBC cell line Bu68.8, BCG (MOI = 1), or medium alone for 4 days. Repartition of ILC1, ILC2 and ILC3 subsets as well as frequencies of total ILC are shown for each indicated conditions. (E) ILC2 frequencies in post-BCG urine samples from patients with high (T/Mhi, n = 10) or low (T/Mlo, n = 13) T cell/MDSC ratios, as identified in Figure 2, were compared (2-tailed Student’s t test). ILC2 frequencies were correlated with the T cell/MDSC ratios (F) as well as T cell (G) and M-MDSC (H) frequencies in corresponding urine samples. Lines indicate linear regression. Spearman’s rank correlation coefficients (R) and the corresponding P values are indicated in F–H. *P < 0.05, **P < 0.01, and ****P < 0.0001, by 1-way ANOVA followed by Tukey’s test (B), a post test for linear trend (C), or Dunnett’s test (D) for comparison of subsets, longitudinal changes, or conditions versus medium, respectively.

Article Snippet: The following mAbs were used at predetermined optimal concentrations: anti–CD45-phycoerythrin (PE) (2D1); anti–CD11b-allophycocyanin (APC)/eFluor 780 (ICRF44); anti–CD3-PerCP/Cy5.5 (SK7); anti–FcεR1α-FITC (AER-37, CRA1); anti–CD3-PE/Cy7 (UCHT1); anti–CD14-PE/Cy7 (61D3); anti–CD19-PE/Cy7 (SJ25C1); anti–CD303a-PE/Cy7 (201A) (all from eBioscience); anti–CD14–Pacific Blue (HCD14); anti–CD15-PerCP/Cy5.5 (W6D3); anti–CD33-APC (WM53); anti–HLA-DR−PE/Cy7 (L243); anti–CD15−Pacific Blue (W6D3); anti–CD56-PE/Cy7 (HCD56); anti–CD117 (c-Kit)-PE (104D2); anti-CD294(CRTH2)-PerCP/Cy5.5 (BM16); anti–CD11c-PE/Cy7 (Bu15); anti–CD15-PE/Cy7 (W6D3); anti–CD163-PE (GHI/61); anti–CD64-PerCP/Cy5.5 (10.1); and anti–CD68-AF647 (Y1/82A) (all from BioLegend); anti–CD3-PE/AF610 (7D6); anti–CD19-PE/AF610 (SJ25C1); anti–CD56−PE/Texas Red (MEM-188); and anti–CD8-PE/AF610 (3B5) (all from Invitrogen, Thermo Fisher Scientific); anti–CD4-APC/H7 (RPA-T4); anti–CD127-AF647 (HIL-7R-M21); anti–CD203c-BV421 (NP4D6); anti–CD80-APC/H7 (L307); anti–CD115(CSF-1R)-BB515 (9-4D2); and anti–CCR4-PE/Cy7 (1G1) (all from BD Biosciences); and anti–IL-13Rα1–PE (419718) (from R&D Systems).

Techniques: Labeling

RSV-induced airway immune infiltration is reduced in TLR7 KO mice. WT C57BL/6 or TLR7 KO mice were infected with RSV (0.5-2x10 7 PFUs) or PBS via intranasal administration and analysis performed after 4, 7 or 42 dpi. (A) Airway inflammation was assessed by counting the total number of live cells isolated from the bronchoalveolar lavage (BAL) fluid. Data are expressed as mean ± SEM, n = 12-16 mice per experimental group from three independent experiments. Immune cell populations in the airways collected in the BAL fluid were determined by flow cytometry. (B) Innate immune cell types: interstitial macrophages (CD11b + F4/80 + ), patrolling monocytes (CD11b + Ly6C lo ), inflammatory monocytes (CD11b + Ly6C hi ), NK cells (CD3 - NK1.1 + ), neutrophils (CD11b + Ly6G + ), plasmacytoid DCs (CD11c + CD11b - PDCA-1 + ) and myeloid DCs (CD11c + CD11b + MHCII + ). Numbers of differentially stained eosinophils was determined by counting 500 cells from random fields by standard morphological criteria relative to the total number of isolated cells. (C) Adaptive T cell subtypes: T helper (CD3 + CD4 + ) and cytotoxic T cells (CD3 + CD8 + ). Cell populations were measured as absolute number of CD45 + population per total live cells. Data are expressed as mean ± SEM, n = 5-8 mice per experimental group from two independent experiments. Statistical analysis was conducted using two-way ANOVA test followed by Tukey’s post hoc test for multiple comparison test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001).

Journal: Frontiers in Immunology

Article Title: TLR7 promotes chronic airway disease in RSV-infected mice

doi: 10.3389/fimmu.2023.1240552

Figure Lengend Snippet: RSV-induced airway immune infiltration is reduced in TLR7 KO mice. WT C57BL/6 or TLR7 KO mice were infected with RSV (0.5-2x10 7 PFUs) or PBS via intranasal administration and analysis performed after 4, 7 or 42 dpi. (A) Airway inflammation was assessed by counting the total number of live cells isolated from the bronchoalveolar lavage (BAL) fluid. Data are expressed as mean ± SEM, n = 12-16 mice per experimental group from three independent experiments. Immune cell populations in the airways collected in the BAL fluid were determined by flow cytometry. (B) Innate immune cell types: interstitial macrophages (CD11b + F4/80 + ), patrolling monocytes (CD11b + Ly6C lo ), inflammatory monocytes (CD11b + Ly6C hi ), NK cells (CD3 - NK1.1 + ), neutrophils (CD11b + Ly6G + ), plasmacytoid DCs (CD11c + CD11b - PDCA-1 + ) and myeloid DCs (CD11c + CD11b + MHCII + ). Numbers of differentially stained eosinophils was determined by counting 500 cells from random fields by standard morphological criteria relative to the total number of isolated cells. (C) Adaptive T cell subtypes: T helper (CD3 + CD4 + ) and cytotoxic T cells (CD3 + CD8 + ). Cell populations were measured as absolute number of CD45 + population per total live cells. Data are expressed as mean ± SEM, n = 5-8 mice per experimental group from two independent experiments. Statistical analysis was conducted using two-way ANOVA test followed by Tukey’s post hoc test for multiple comparison test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001).

Article Snippet: The following Biolegend antibodies were used (unless stated otherwise): CD45-PerCP (30-F11), CD3e-APC (145-2C11; BD Pharmingen), CD4-BV605 (RM4-5), CD8a-PE-Cy7 (53-6.7), NK1.1-FITC (PK136), CD11b-BV421 (M1/70), CD11c-PE-Cy7 (N418; eBioscience), PDCA-1-PE (JF05-1C2.4.1; Miltenyi Biotec), MHC-II-APC (M5/114.15.2), Ly6C-FITC (HK1.4), Ly6G-APC-Cy7 (1A8), F4/80-PE (BM8; eBiocience), iNOS-FITC (6; BD Transductions Laboratories) and CD206-PE-Cy7 (MMR; eBioscience).

Techniques: Infection, Isolation, Flow Cytometry, Staining, Comparison

RSV-induced lung infiltration of innate immune cells is reduced in TLR7 KO mice. WT C57BL/6 or TLR7 KO mice were infected with RSV (0.5-2x10 7 PFUs) or PBS via intranasal administration. Immune cell populations in lung tissue were determined by flow cytometry after 4, 7 or 42 dpi. (A) Innate immune cell types: interstitial macrophages (CD11b + F4/80 + ), patrolling monocytes (CD11b + Ly6C lo ), inflammatory monocytes (CD11b + Ly6C hi ), NK cells (CD3 - NK1.1 + ), neutrophils (CD11b + Ly6G + ), plasmacytoid DCs (CD11c + CD11b - PDCA-1 + ) and myeloid DCs (CD11c + CD11b + MCHCII + ). (B) Adaptive T cell types: T helper (CD3 + CD4 + ) and cytotoxic T cells (CD3 + CD8 + ). Cell populations are measured as absolute number of CD45 + population per 10,000 counting beads. Data are expressed as mean ± SEM, n = 5-8 mice per experimental group from two independent experiments. Statistical analysis was conducted using two-way ANOVA test followed by Tukey’s post hoc test for multiple comparison test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001).

Journal: Frontiers in Immunology

Article Title: TLR7 promotes chronic airway disease in RSV-infected mice

doi: 10.3389/fimmu.2023.1240552

Figure Lengend Snippet: RSV-induced lung infiltration of innate immune cells is reduced in TLR7 KO mice. WT C57BL/6 or TLR7 KO mice were infected with RSV (0.5-2x10 7 PFUs) or PBS via intranasal administration. Immune cell populations in lung tissue were determined by flow cytometry after 4, 7 or 42 dpi. (A) Innate immune cell types: interstitial macrophages (CD11b + F4/80 + ), patrolling monocytes (CD11b + Ly6C lo ), inflammatory monocytes (CD11b + Ly6C hi ), NK cells (CD3 - NK1.1 + ), neutrophils (CD11b + Ly6G + ), plasmacytoid DCs (CD11c + CD11b - PDCA-1 + ) and myeloid DCs (CD11c + CD11b + MCHCII + ). (B) Adaptive T cell types: T helper (CD3 + CD4 + ) and cytotoxic T cells (CD3 + CD8 + ). Cell populations are measured as absolute number of CD45 + population per 10,000 counting beads. Data are expressed as mean ± SEM, n = 5-8 mice per experimental group from two independent experiments. Statistical analysis was conducted using two-way ANOVA test followed by Tukey’s post hoc test for multiple comparison test (*p < 0.05, **p < 0.01, ***p<0.001, ****p<0.0001).

Article Snippet: The following Biolegend antibodies were used (unless stated otherwise): CD45-PerCP (30-F11), CD3e-APC (145-2C11; BD Pharmingen), CD4-BV605 (RM4-5), CD8a-PE-Cy7 (53-6.7), NK1.1-FITC (PK136), CD11b-BV421 (M1/70), CD11c-PE-Cy7 (N418; eBioscience), PDCA-1-PE (JF05-1C2.4.1; Miltenyi Biotec), MHC-II-APC (M5/114.15.2), Ly6C-FITC (HK1.4), Ly6G-APC-Cy7 (1A8), F4/80-PE (BM8; eBiocience), iNOS-FITC (6; BD Transductions Laboratories) and CD206-PE-Cy7 (MMR; eBioscience).

Techniques: Infection, Flow Cytometry, Comparison